top of page
Innovative single cell culture
Single cell capture and culture slide
CellGem is a simple and smart single cell capture and culture platform. It utilizes microfluidic technology which enables precise cell manipulation at high-throughput and small reagent consumption.
CellGem can be used for
Stable cell line development
- Monoclonal antibody production
- Gene transfection/editing
Cell heterogeneity studies
- Cancer stem cells
Principle
The CellGem platform comprises of an array of microwells for capturing single cells, coupled with a corresponding array of culture wells for long-term culture of the single cells.
Validated cell line
-
Cho-k1
-
AA8
-
Jurkat
-
SP2/0-Ag14
-
A-549
-
L929
-
Eh7a
Benefits
-
High single cell efficiency
-
High cell viability
-
Reduce sample consumption
-
Easy operation (only a standard pipette needed)
-
Easy observation
-
High cells growth rate
CellGem
Video
-
Q：An air bubble was trapped in the chip during the priming step. How do I get rid of it?A： Fully aspirate the priming solution from the chip, then rapidly inject the priming solution back into the chip. This helps to remove bubbles.
-
Q：Is there a contamination risk of using sealing tape to seal the chip inlets?A： The sealing tape used in the kit is tested and certified to be sterile. It's safe to use with cell culture.
-
Q：After flipping over the chip, how do the captured cells accurately land in their corresponding culture wells?A： The capture wells are specifically designed to ensure that each captured single cell will only land in its designated culture well after flipping the chip. However, the chip can only be flipped over once. Users should avoid flipping it over again after the single cell transfer step.
-
Q：What are the advantages of CellGem® compared to other single cell separation products in the market?A： CellGem® does not require complex instrumentation and is easy to use. It also has high single-cell capture efficiency, high single-cell growth rate, easy to identify single cells, and consumes minimal cell culture medium.
-
Q：Following the previous question, what do you mean by high single cell growth rate? In theory, single cells don’t grow into colonies efficiently, why is this?A： Because all the cells inside the CellGem® chip share the same pool of culture medium, and also share secreted growth factors between each other. Each single cell still owns its isolated growth space at the same time.(Depend on cell type.)
-
Q：Why is my cell capture efficiency low? How do I increase single cell capture efficiency?A： 1. If your cells are clumpy, then they cannot be efficiently captured by the capture wells. We recommend trying a cell dissociation reagent that's compatible with your cells (ex. Accumax, sorting buffer.) 2. There may be excess bubbles in the chip, affecting cell capture efficiency. We recommend trying the bubble removal method in page 8 of the handbook (Page 7) 3. You may have selected a capture well size that's not a good fit for your cell of interest. We recommend measuring the size (diameter) of your cells and selecting the product with the corresponding suitable capture well size(Page 6) 4. Your cell concentration may be too low. Cell concentration of 1*106 cell/ml or above is recommended. 5. Loading cells multiple times to increase capture efficiency.
-
Q：If there are cells existing in the channel, will it affect the monoclonality of my single cell-derived colony? If so, how do I perform the subsequent cell harvesting steps to avoid contaminating my single cell colonies?A： Cells exist in the channel do have the potential of contaminating your single cell colonies and affecting monoclonality. So, It's important to ensure that the washing step is done well to remove excess cells from the chip. If residual cells still exist in the channel, do not directly inject trypsin into the chip before harvesting stage. Instead, disassemble the chip while the cells are still attached, then individually add trypsin to each culture well to detach the cells. The working volume of each culture well is 1.5 μL.
bottom of page